Antioxidant Effect of Naringin Demonstrated through a Bayes’ Theorem Driven Multidisciplinary Approach Reveals its Prophylactic Potential as a Dietary Supplement for Ischemic Stroke

Protein targets of Naringin were acquired from the following databases using filters like target organism - Homo sapiens, Rattus norvegicus, Mus musculus, and target type - single protein. The standard human gene IDs corresponding to the ChEMBL/Uniprot ID/Accession number for the targets were mapped using the ID-mapping feature in Uniprot. Duplicates were eliminated. This created a data vector of length 289 which will serve as the prior probability vector for bayesian analysis

    The 289 protein targets were ranked using Bayes' theorem by sequentially integrating multiple proteomics datasets based on the following assumptions (colour-coded):
  1. The protein target must be expressed (transcribed and translated) in the target brain cell type (i.e., neuron - N2a cells) to play a protective role following Naringin administration.
  2. The neuronal proteins that are perturbed following OGD stress are more likely to be involved directly or indirectly with the Naringin effect.
  3. Naringin is widely reported to have antioxidant effects. ROS-associated proteome is likely to mediate the antioxidant action of Naringin in brain cells.